Interesting Bioinformatics Articles
Following is a collection of articles which I feel every Bioinformatician must be aware of. I will keep updating this list from time to time -
Screen command in UNIX
Screen is a very useful command to have in your toolbox if you frequently use interactive sessions on your supercomputer logged in through a VPN. A VPN typically has a time limit, and you may get disconnected from it without any warning when you have poor internet connection. Screen program
Types of models in DESeq2
There are 2 major types of regression models one can specify in DESeq2 to explore the raw count matrices from an RNA-seq experiment - * Mean-reference model for Factors * Regression model for Covariates Mean-reference model for Factors - Factors typically represent categorical variable such as Gender, Ethnicity, Race etc. The mean-reference
Steps in DESeq function
For any RNA-seq data analysis project, one would most probably end up using the R package DESeq2. The function 'DESeq' is the main function that is called to obtain the differentially expressed genes. But what exactly does this function do? In short, DESeq function combines the following 3 steps -